三氯乙烯致敏豚鼠肝脏损害研究

doi:10.3969/j.issn.1004-616x.2011.05.005

›› 2011, Vol. 23 ›› Issue (5): 344-348.doi: 10.3969/j.issn.1004-616x.2011.05.005

三氯乙烯致敏豚鼠肝脏损害研究

朱启星,徐辉,冷静沈彤

1. 安徽医科大学公共卫生学院劳动卫生与环境卫生系，安徽合肥 230032；2. 安徽医科大学皮肤病研究所，安徽合肥 230032

收稿日期:2011-05-23 修回日期:2011-07-07 出版日期:2011-09-30 发布日期:2011-09-30
通讯作者: 朱启星

Liver damage induced by trichloroethylene in sensitized guinea pigs

ZHU Qi-xing，XU Hui，LENG Jing，SHEN Tong

1．Department of Occupational and Environmental Hygiene, School of Public Health, Anhui Medical University, Hefei 230032; 2．Institute of Dermatology，Anhui Medical University, Hefei 230032, Anhui, China

Received:2011-05-23 Revised:2011-07-07 Online:2011-09-30 Published:2011-09-30
Contact: ZHU Qi-xing

摘要/Abstract

摘要： 目的：观察三氯乙烯 (trichloroethylene，TCE)致敏豚鼠的肝脏功能改变和超微结构变化。方法：选用体质量250~300 g的白色雌性豚鼠，随机分成空白对照组，溶剂 (橄榄油)对照组，TCE处理组(包括TCE致敏24 h、72 h组和TCE未致敏24 h、72 h组)。根据豚鼠最大值试验 (guinea pig maximization test，GPMT)法进行处理，在终末激发后24 h和72 h心脏采血，用全自动生化分析仪测定血清中谷丙转移酶(ALT)、谷草转移酶(AST)、总蛋白 (TP)、白蛋白 (ALB)、球蛋白 (GLB)、白球比值 (A/G)等肝功能指标，无菌条件下取肝组织进行病理学和超微结构检查。结果：TCE 处理组致敏率为65.38%。TCE致敏72 h组ALT和AST水平比TCE致敏24 h组和TCE未致敏72 h组均升高 (P均<0.05)；TCE致敏72 h组ALB水平比溶剂对照组和TCE未致敏72 h组降低 (P<0.05)。病理检查显示TCE致敏72 h组中见到较多肝细胞水肿，并有胞核破裂消失。透射电镜观察显示TCE未致敏组可见少数线粒体空泡、变性，粗面内质网减少；TCE致敏组肝脏细胞中线粒体空泡、变性、数量减少，粗面内质网断裂、扩张、糖原颗粒明显减少；且在末次激发后72 h组比24 h组的损害更严重。结论：TCE处理组豚鼠体内发生了肝脏功能障碍和超微结构的破坏，且损伤的程度随着时间的延长而加重。

关键词: 三氯乙烯, 豚鼠, 肝脏, 损害

Abstract: OBJECTIVE: To observe the changes of liver function and ultrastructures induced by trichloroethylene in sensitized guinea pigs. METHODS： White female guinea pigs (250-300 g) were randomly divided into blank control group， solvent (olive oil) control group and TCE treatment group. Guinea pigs were treated with guinea pig maximization test (GPMT). Specimens were collected at 24 h and 72 h after last stimulation， liver tissue pathology and ultrastructures examined，and AST，ALT，TP，ALB，GLB and A/G in serum measured. RESULTS： Sensitization rates were 65.38% in TCE treatment groups. Compared with TCE sensitized 24 h group and TCE non-sensitized 72 h group， ALT and AST levels in TCE sensitized 72 h group increased significantly(P<0.05). Compared with solvent group and TCE non-sensitized 72 h group， ALB levels in TCE sensitized 72 h group increased significantly(P<0.05). TCE sensitized group 72 h revealed more edema in liver cells with nucleus ruptured and disintegrated. TEM observation showed： a few mitochondria vacuoles， degeneration， rough endoplasmic reticulum decreased in TCE non-sensitized group. TCE sensitized group demonstrated reduction in the number of mitochondria in liver cells， rough endoplasmic reticulum fracture， expansion， decreased glycogen granules. In the group of 72 h after the last challenge，more serious damage was visible than those in 24 h group. CONCLUSION： Liver dysfunction and ultrastructural damage occurred in sensitized guinea pigs induced by trichloroethylene. The extent of injury gradually increase with time.

Key words: trichloroethylene, guinea pigs, liver, injury

朱启星,徐辉,冷静沈彤. 三氯乙烯致敏豚鼠肝脏损害研究[J]. , 2011, 23(5): 344-348.

ZHU Qi-xing，XU Hui，LENG Jing，SHEN Tong. Liver damage induced by trichloroethylene in sensitized guinea pigs[J]. , 2011, 23(5): 344-348.

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三氯乙烯致敏豚鼠肝脏损害研究

Liver damage induced by trichloroethylene in sensitized guinea pigs

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